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Key Features

PE/Cyanine5 Anti-Mouse Ly6A/E (Sca-1) Antibody [D7] (AGEL2354)

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SKU AGEL2354
Product Type Antibody
Antibody Type Monoclonal Antibody
Applications FCM
Reactivity Mouse
Host Species Rat
Isotype Rat IgG2a, κ
Clone D7
Conjugation PE/Cyanine5
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Description

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PE/Cyanine5 Anti-Mouse Ly6A/E (Sca-1) Antibody [D7]

The PE-Cyanine5 Anti-Mouse Ly6A/E (Sca-1) Antibody (D7) is a valuable tool for research involving the Ly6A/E (Sca-1) protein, a marker of stem cells and immune cells. This antibody, specifically designed for use with mouse samples, is raised in a host organism that enhances its reactivity and specificity. Validated for applications such as flow cytometry and immunofluorescence, it enables precise detection and analysis of Ly6A/E (Sca-1) expression in different cell populations.Ly6A/E (Sca-1) is a surface marker with diverse functions in stem cell biology and immune cell regulation.

Its involvement in processes such as self-renewal and differentiation makes it a key target for studies in regenerative medicine, cancer biology, and immunology. By understanding the expression and role of Ly6A/E (Sca-1) in various cell types and disease contexts, researchers can unravel important insights into cell behavior, tissue regeneration, and immune responses. This antibody provides a powerful tool to facilitate in-depth investigations into the function and significance of Ly6A/E (Sca-1) in diverse biological systems.

Product Title:PE/Cyanine5 Anti-Mouse Ly6A/E (Sca-1) Antibody [D7]
SKU:AGEL2354
Size:25µg, 100µg
Clone No:D7
Synonyms:Ly6a;Ly6;Ly-6A.2/Ly-6E.1;SCA-1;TAP;
Host Species:Rat
Reactivity:Mouse
Application:FCM
Isotype:Rat IgG2a, κ
Isotype Control:PE/Cyanine5 Rat IgG2a, κ Isotype Control[2A3]
Form:Liquid
Conjugation:PE/Cyanine 5
Recommended Use:Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:Spectrum Image
Swissprot:P05533
Gene ID:110454
Storage Buffer:Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

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