The PGI2 (Prostacyclin) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of prostacyclin levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an invaluable tool for researchers in various fields.Prostacyclin, also known as PGI2, is a key mediator in inflammation and vascular health. It plays a crucial role in maintaining cardiovascular homeostasis and regulating blood flow, making it a promising therapeutic target for cardiovascular diseases.
By accurately measuring prostacyclin levels, researchers can gain insights into the mechanisms underlying these conditions and develop targeted treatment strategies.In conclusion, the PGI2 (Prostacyclin) ELISA Kit is a valuable resource for researchers seeking to investigate the role of prostacyclin in various biological processes. Its high sensitivity and specificity ensure precise and reliable results, making it an indispensable tool for studying cardiovascular health and developing innovative therapeutic interventions.
Product Name:
PGI2 (Prostacyclin) ELISA Kit
SKU:
UNES00016
Target:
PGI2 (Prostacyclin) (UNES00016)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGI2. During the reaction, PGI2 in the sample or standard competes with a fixed amount of PGI2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGI2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGI2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
88-101
93-109
Average (%)
105
94
101
1:4
Range (%)
94-108
86-98
94-107
Average (%)
101
91
100
1:8
Range (%)
101-114
90-102
92-105
Average (%)
108
96
99
1:16
Range (%)
100-115
90-103
92-110
Average (%)
106
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.64
246.44
963.63
90.56
267.93
1038.28
Standard deviation
5.82
11.68
44.91
5.8
15.86
39.56
C V (%)
6.15
4.74
4.66
6.4
5.92
3.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of PGI2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes PGI2 in samples. No significant cross-reactivity or interference between PGI2 and analogues was observed.
