The Phospho-AKT1S1/PRAS40-T246 Antibody (CABP0793) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It binds specifically to the phosphorylated form of Akt1S1, allowing for the detection and analysis of this important signaling molecule in various cell types.Akt1S1, a key component of the Akt signaling pathway, plays a crucial role in cell growth, proliferation, and survival. Phosphorylation of Akt1S1 at threonine 246 is known to regulate its activity and downstream signaling, making it a critical target for research in cancer, diabetes, and other diseases where Akt signaling is dysregulated.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-AKT1S1/PRAS40-T246 Antibody
SKU:
CABP0793
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
LNTS D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Lobe, PRAS40, Phospho-AKT1S1/PRAS40-T246
Positive Sample:
HeLa treated with Insulin
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
27kDa
Observed MW:
40kDa
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).
Purification Method
Affinity purification
Gene ID
84335
RRID
AB_2770905
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-AKT1S1/PRAS40-T246 Rabbit pAb (CABP0793) at 1:400 dilution. HeLa cells were treated with Insulin (100 nM) at 37℃ for 5 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 45s.
