The Phospho-CDK6 (Y24) Polyclonal Antibody (CABP0289) is a valuable tool for researchers studying CDK6, a key regulator of cell cycle progression. This antibody, produced in rabbits, exhibits high specificity for phosphorylated CDK6 at tyrosine 24 and is validated for use in Western blot applications. By specifically targeting this phosphorylation site, researchers can gain insight into the activation and function of CDK6 in various biological contexts.CDK6 is a crucial player in cell cycle control, promoting cell proliferation and growth. Phosphorylation at tyrosine 24 is known to regulate the activity of CDK6, influencing its ability to phosphorylate downstream targets and drive cell cycle progression.
By studying this specific phosphorylation event, researchers can uncover the mechanisms by which CDK6 is regulated and its role in diseases like cancer, where aberrant cell cycle regulation is a hallmark.Overall, the Phospho-CDK6 (Y24) Polyclonal Antibody (CABP0289) provides a valuable tool for elucidating the role of CDK6 in cell cycle regulation and disease, making it a crucial asset for studies in cancer biology and therapeutic development.
Product Name:
Phospho-CDK6-Y24 Rabbit Polyclonal Antibody
SKU:
CABP0289
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y24 of human CDK6
Tested Applications:
WBIF/ICC
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:100 - 1:200
Synonyms:
MCPH12; PLSTIRE; Phospho-CDK6-Y24
Positive Sample:
293
Conjugate:
Unconjugated
Cellular Localization:
Cell projection, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, ruffle.
Calculated MW:
37kDa
Observed MW:
37kDa
The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.
Purification Method:
Affinity purification
Gene ID:
1021
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells using Phospho-CDK6-Y24 Rabbit pAb (CABP0289).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
