The Phospho-EGFR-Y1068 Monoclonal Antibody (CABP0994) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically recognizes the phosphorylated form of EGFR at the Y1068 site and is validated for use in various applications, including Western blot and immunohistochemistry.EGFR is a transmembrane protein that plays a crucial role in a variety of cellular processes, including cell proliferation, survival, and differentiation. Phosphorylation of EGFR at the Y1068 site is known to activate downstream signaling pathways involved in cell growth and survival, making this antibody an essential tool for studying EGFR signaling in cancer and other diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-EGFR-Y1068 Monoclonal Antibody
SKU:
CABP0994
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1538
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method
Affinity purification
Gene ID
1956
RRID
AB_2863886
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from BxPC-3 cells, using Phospho-EGFR-Y1068 Rabbit mAb (CABP0994) at 1:1000 dilution. BxPC-3 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
