The Phospho-EGFR-Y1148 Antibody (CABP0028) is a high-quality antibody developed for reliable detection and analysis of target proteins. EGFR is a key player in cell growth, proliferation, and survival, and dysregulation of its signaling pathways is implicated in various diseases, including cancer.This antibody, produced in rabbits, is highly specific for detecting phosphorylated EGFR at tyrosine 1148 in human samples. It has been validated for use in Western blot applications, allowing for the precise detection and quantification of phosphorylated EGFR in different cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-EGFR-Y1148 Antibody
SKU:
CABP0028
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PDYQ Q
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Endoplasmic Reticulum Membrane, Endosome, Endosome Membrane, Golgi Apparatus Membrane, Nucleus Membrane, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
134kDa
Observed MW:
180kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method
Affinity purification
Gene ID
1956
RRID
AB_2771052
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-EGFR-Y1148 Rabbit pAb (CABP0028) at 1:1000 dilution. A431 cells were treated with EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
