The Phospho-ErbB4/HER4-Y1284 Antibody (CABP0034) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit hosts, demonstrates high specificity and sensitivity towards phosphorylated ERBB4 at tyrosine 1284 in human samples, making it ideal for Western blot applications.The phosphorylation of ERBB4 at tyrosine 1284 plays a vital role in signaling pathways related to cell proliferation, survival, and differentiation. By targeting this specific phosphorylation site, researchers can gain insights into the function and activation of ERBB4 in various cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-ErbB4/HER4-Y1284 Antibody
SKU:
CABP0034
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PEYL S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HER4, ALS19, p180erbB4, Phospho-ErbB4/HER4-Y1284
Positive Sample:
MCF7 treated with IGF
Cellular Localization:
Cell Membrane, Mitochondrion, Nucleus, Single-Pass Type I Membrane Protein.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Purification Method
Affinity purification
Gene ID
2066
RRID
AB_2771096
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells using Phospho-ErbB4/HER4-Y1284 Rabbit pAb (CABP0034) at 1:700 dilution. MCF7 cells were treated with IGF (200 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 20 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
