The Phospho-GAB1-Y307 Polyclonal Antibody (CABP0961) is a high-quality antibody developed for reliable detection and analysis of target proteins. IRS (Insulin Receptor Substrate) is a member of the IRS1-like multisubstrate docking protein family that serves as an important mediator of branching tubulogenesis. It plays a central role in cellular growth responses, oncogenic transformation, and apoptosis, making it a key node in growth factor and insulin signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-GAB1-Y307 Polyclonal Antibody
SKU:
CABP0961
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DFNB26, Phospho-GAB1-Y307
Positive Sample:
NIH/3T3 treated with IGF-1
Cellular Localization:
Cell-Cell Junction, Cytoplasm, Cytosol.
Calculated MW:
77kDa
Observed MW:
115kDa
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2549
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-GAB1-Y307 Rabbit pAb (CABP0961) at 1:1000 dilution. NIH/3T3 cells were treated with IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
