The Phospho-HDAC2-S394 Polyclonal Antibody (CABP0201) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It targets the phosphorylated form of HDAC2 at Serine 394, allowing for precise detection and analysis in various cell types.HDAC2, a member of the histone deacetylase family, is involved in chromatin remodeling and gene expression regulation. Phosphorylation of HDAC2 at S394 has been linked to its activity in various cellular processes, including cell cycle regulation and DNA damage response.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-HDAC2-S394 Polyclonal Antibody
SKU:
CABP0201
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:100 - 1:500
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HD2, RPD3, YAF1, KDAC2, Phospho-HDAC2-S394
Positive Sample:
HepG2
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
55kDa
Observed MW:
62kDa
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3066
RRID
AB_2771159
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using Phospho-HDAC2-S394 Rabbit pAb (CABP0201) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human testis using Phospho-HDAC2-S394 Rabbit pAb (CABP0201) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
