The Phospho-Histone H4-S1 Antibody (CABP0901) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets Histone H4, a key protein involved in packaging DNA into chromatin, and detects phosphorylation at Serine 1. Histone H4 phosphorylation at Serine 1 is known to play a role in gene expression regulation, cell cycle progression, and DNA damage response. By using this antibody in Western blot applications, researchers can gain valuable insights into the dynamic regulation of chromatin structure and function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Phospho-Histone H4-S1 Antibody
SKU:
CABP0901
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MSGR
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method
Affinity purification
Gene ID
8359
RRID
AB_2863819
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (CABP0901) at 1:1000 dilution or Histone H4 Rabbit pAb (CAB1131). NIH/3T3 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or Nocodazole (50 ng/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
