The Phospho-Lck-Y505 Antibody (CABP0285) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically recognizes phosphorylation at tyrosine 505 of the Lck protein.Lck is a critical regulator of T cell activation and function, with phosphorylation at Y505 known to influence its activity and downstream signaling cascades. By targeting this phosphorylation site, the CABP0285 antibody allows for the detection and analysis of activated Lck in cell lysates, serum samples, and other biological fluids.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Lck-Y505 Antibody
SKU:
CABP0285
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GQYQ P
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
Purification Method
Affinity purification
Gene ID
3932
RRID
AB_2771264
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-Lck-Y505 Rabbit pAb (CABP0285) at 1:1000 dilution. Jurkat cells were treated with Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes or treated with Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 1s.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg Phospho-Lck-Y505 pAb (CABP0285). Western blot was performed from the immunoprecipitate using Phospho-Lck-Y505 pAb (CABP0285) at a dilution of 1:1000. Jurkat cells were treated with Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.
