The Phospho-MEK1-S298 Antibody (CABP0063) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, demonstrates high specificity and sensitivity for detecting phosphorylation of MAP2K1 at serine 298 in human samples. Validated for use in Western blot applications, this antibody enables precise detection and quantification of phosphorylated MAP2K1 in various cell types.MAP2K1, also known as MEK1, is a key kinase that regulates important cellular processes such as cell growth, differentiation, and survival. Phosphorylation of MAP2K1 at serine 298 is a crucial post-translational modification that modulates its activity and downstream signaling cascades.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-MEK1-S298 Antibody
SKU:
CABP0063
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PLSS Y
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with ATP, C2C12 treated with ATP, C6 treated with ATP
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
43kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method
Affinity purification
Gene ID
5604
RRID
AB_2771281
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (CABP0063) at 1:1000 dilution or MEK1 antibody (CAB12687). HeLa cells were treated with PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight or treated with ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit pAb (CABP0063) at 1:1000 dilution. C2C12 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with ATP(5 mM) at 30℃ for 1 hour. C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
