The Phospho-MEK1-S298 Monoclonal Antibody (CABP1020) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot and immunohistochemistry applications. It specifically binds to the phosphorylated form of MEK1 at serine 298, enabling accurate detection and analysis in various cell types.MEK1 is a key kinase in the MAPK signaling pathway, playing a crucial role in cell proliferation, differentiation, and survival. Phosphorylation of MEK1 at serine 298 is known to modulate its activity, leading to downstream activation of ERK and other signaling molecules.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-MEK1-S298 Monoclonal Antibody
SKU:
CABP1020
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1565
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PLSS Y
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEL, Phospho-MEK1-S298
Positive Sample:
HeLa, C6, HeLa treated with PMA, C6 treated with PMA
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral Membrane Protein, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole Body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method
Affinity purification
Gene ID
5604
RRID
AB_2863905
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-MEK1-S298 Rabbit mAb (CABP1020) at 1:1000 dilution. Both HeLa cells and C6 cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour or treated with PMA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
