The Phospho-Met-Y1234/Y1235 Monoclonal Antibody (CABP1339) is a high-quality antibody developed for reliable detection and analysis of target proteins. -
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Product Name:
Phospho-Met-Y1234/Y1235 Monoclonal Antibody
SKU:
CABP1339
Size:
100μL, 20μL
Tested Applications:
-
Recommended Dilution:
Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 Rabbit mAb (CABP1339) at1:2000 dilution. HeLa cells were treated with HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of various lysates, using Phospho-Met-Y1234/Y1235 Rabbit mAb (CABP1339) at1:2000 dilution. C2C12 and C6 cells were treated with HGF (40 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
