The Phospho-NDRG1-S330 Antibody (CABP0807) is a high-quality antibody developed for reliable detection and analysis of target proteins. NDRG1 is a protein involved in various cellular processes, including cell growth, differentiation, and stress response. The antibody, produced in rabbits, is highly specific to the phosphorylated form of NDRG1 at serine 330, making it ideal for use in Western blot applications.Phosphorylation at serine 330 has been linked to the regulation of NDRG1 activity and its involvement in signaling pathways related to cell proliferation and survival. By targeting this specific phosphorylation site, researchers can gain insights into the molecular mechanisms underlying these cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-NDRG1-S330 Antibody
SKU:
CABP0807
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TASG S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
10397
RRID
AB_2771355
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-NDRG1-S330 Rabbit pAb (CAB2142). Jurkat cells were treated with Calyculin A (100nM) for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
