The Phospho-eNOS-S1177 Antibody (CABP0421) is a high-quality antibody developed for reliable detection and analysis of target proteins. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. RRID AB_2771372 Gene ID 4846 Swiss Prot Synonym eNOS; ECNOS; Phospho-eNOS-S1177
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-eNOS-S1177 Antibody
SKU:
CABP0421
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
eNOS, ECNOS, Phospho-eNOS-S1177
Positive Sample:
Jurkat treated with Calyculin A, HeLa treated with IGF-1
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. RRID AB_2771372 Gene ID 4846 Swiss Prot Synonym eNOS; ECNOS; Phospho-eNOS-S1177
Purification Method:
Affinity purification
Gene ID:
4846
RRID:
AB_2771372
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-eNOS-S1177 Rabbit pAb (CABP0421). Jurkat cells were treated with Calyculin A. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Western blot analysis of lysates from HeLa cells, using Phospho-eNOS-S1177 Rabbit pAb (A1548). HeLa cells were treated with IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
