The Phospho-p53-S33 Monoclonal Antibody (CABP0984) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been rigorously validated for Western blot applications.The phosphorylation of p53 at serine 33 is known to influence its stability, DNA-binding activity, and transcriptional regulation, ultimately impacting important cellular processes such as DNA repair and cell death pathways. By targeting this specific phosphorylation site, researchers can gain insights into the functional role of p53 in response to cellular stress, DNA damage, and oncogenic signals.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-p53-S33 Monoclonal Antibody
SKU:
CABP0984
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1528
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VLSP L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method
Affinity purification
Gene ID
7157
RRID
AB_2863878
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit mAb (CABP0984) at 1:1000 dilution. HT-29 cells were treated with nocodazole (100 ng/mL) at 37℃ for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
