The Phospho-p53-S9 Monoclonal Antibody (CABP0985) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, has high specificity and sensitivity for human samples, and has been validated for use in Western blot applications. By binding to the phosphorylated form of p53 at serine 9, this antibody enables the detection and analysis of p53 activation in various cell types, making it an essential tool for studies in cancer biology, DNA damage response, and cell signaling pathways.Phosphorylation of p53 at serine 9 is known to influence its stability, activity, and ability to induce cell cycle arrest or apoptosis in response to cellular stress.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-p53-S9 Monoclonal Antibody
SKU:
CABP0985
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC1529
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
DPSV E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method
Affinity purification
Gene ID
7157
RRID
AB_2863879
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb (CABP0985) at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
