The Phospho-p70 S6 Kinase 1-T421/S424 Monoclonal Antibody (CABP0502) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, developed using rabbits, shows high reactivity with phosphorylated p70 S6 Kinase at threonine 421 and serine 424 residues in human samples, making it ideal for Western blot applications.p70 S6 Kinase is a key regulator of protein synthesis and cell survival, making it a promising target for cancer research and studies on cell signaling pathways. The phosphorylation of p70 S6 Kinase at T421/S424 is known to activate its kinase activity, leading to downstream effects on protein translation and cell growth.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method
Affinity purification
Gene ID
6198
RRID
AB_2863807
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (CABP0502) at 1:1000 dilution. NIH/3T3 and C6 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60S.
Confocal imaging of HeLa cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (CABP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of C6 cells using Phospho-p70 S6 Kinase 1-T421/S424 Rabbit mAb (CABP0502, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007,dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
