Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Recombinant Antibody (RACO0069) is a premium recombinant that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Western Blot Positive WB detected in A549 whole cell lysate(treated with EGF or not) All lanes Phospho-PAK1/PAK2/PAK3 antibody at 1.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65 KDa Observed band size: 65 KDa
IHC image of RACO0069 diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
