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PLK1 Monoclonal Antibody (CAB21082)

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SKU:
CAB21082
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human, Mouse, Rat, Monkey
Host Species:
Rabbit
Synonyms:
PLK, STPK13, PLK1
  • Immunohistochemistry of paraffin-embedded rat spleen using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human tonsil using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using PLK1 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Western blot analysis of extracts of various cell lines, using PLK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using PLK1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Description

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PLK1 Monoclonal Antibody (CAB21082)

The PLK1 Monoclonal Antibody (CAB21082) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through rabbit immunization, offers high specificity and sensitivity for detecting PLK1 in human samples, making it ideal for use in Western blot applications.PLK1, or Polo-like kinase 1, plays a crucial role in cell cycle progression and is often overexpressed in cancer cells, promoting uncontrolled cell growth. By targeting PLK1, researchers hope to develop more effective cancer treatments that inhibit tumor cell proliferation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.

Product Name:PLK1 Monoclonal Antibody
SKU:CAB21082
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat, Monkey
Clone Number:ARC52311
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:KHIN PVAA SLIQ KMLQ TDPT ARPT INEL LNDE FFTS GYIP ARLP ITCL TIPP RFSI APSS LDPS NRKP LTVL NKGL ENPL PERP REKE EPVV RETG EVVD CHLS DMLQ QLHS VNAS KPSE RGLV RQEE AEDP ACIP IFWV SKWV DYSD KYGL GYQL CDNS VGVL FNDS TRLI LYND GDSL QYIE RDGT ESYL TVSS HPNS LMKK ITLL KYFR NYMS EHLL KAGA
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:2000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PLK, STPK13, PLK1
Positive Sample:HeLa, Jurkat, K-562, COS-7, Mouse testis, Rat testis
Cellular Localization:Chromosome, Cytoplasm, Midbody, Nucleus, Centromere, Centrosome, Cytoskeleton, Kinetochore, Microtubule Organizing Center, Spindle.
Calculated MW:68kDa
Observed MW:62kDa

The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.

Purification MethodAffinity purification
Gene ID5347
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using PLK1 Rabbit mAb (CAB21082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageWestern blot analysis of extracts of various cell lines, using PLK1 Rabbit mAb (CAB21082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.

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