The Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Antibody (CABP1164) is a high-quality antibody developed for reliable detection and analysis of target proteins. These proteins play crucial roles in cellular processes, including cell proliferation, cytoskeletal organization, and smooth muscle contraction.This antibody, raised in rabbits, is highly specific and sensitive, making it ideal for use in Western blot and immunohistochemistry applications. It binds specifically to the phosphorylated T696 residue on PPP1R12A, PPP1R12B, and PPP1R12C, allowing for the accurate detection and quantification of these phosphorylated proteins in various experimental settings.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Antibody
SKU:
CABP1164
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RSTQ G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Jurkat treated with Calyculin A
Cellular Localization:
A Band, Actin Cytoskeleton, Centrosome, Contractile Fiber, Cytoplasm, Cytosol, Nucleolus, Nucleoplasm, Plasma Membrane, Z Disc.
Observed MW:
140kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]
Purification Method
Affinity purification
Gene ID
4659 4660 54776
RRID
AB_2864023
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-PPP1R12A/PPP1R12B/PPP1R12C-T696 Rabbit pAb (CABP1164) at 1:1000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
