The Phospho-RB-S780 Monoclonal Antibody (CABP0117) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to detect phosphorylation at serine 780 of the retinoblastoma protein, allowing for precise analysis of signaling pathways involved in cell growth and proliferation.Raised in rabbits, this monoclonal antibody demonstrates high reactivity with human samples and has been validated for use in Western blotting applications. By detecting phospho-Rb at serine 780, researchers can gain insights into the activation or inhibition of cell cycle regulators, providing valuable information for studies in cancer biology, cell signaling, and drug development.
This antibody is validated for use in IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-RB-S780 Monoclonal Antibody
SKU:
CABP0117
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0096
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TLSP IP
Tested Applications:
IHC-PELISA
Recommended Dilution:
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method
Affinity purification
Gene ID
5925
RRID
AB_2863805
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Rat pancreas using Phospho-RB-S780 Rabbit mAb (CABP0117) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-RB-S780 Rabbit mAb (CABP0117) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
