The Phospho-SRC (Y416) Polyclonal Antibody (CABP0480) is specifically designed for research involving the phosphorylated form of SRC, a protein tyrosine kinase involved in cell signaling and regulation. The antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.SRC is a key player in cell growth, differentiation, and survival, making it an important target for cancer research and drug development. The phosphorylation of SRC at tyrosine 416 is known to activate its kinase activity, leading to downstream signaling pathways that promote cell proliferation and migration.
Detection and analysis of phospho-SRC (Y416) is essential for understanding its role in cancer progression and metastasis.This antibody enables researchers to study the phosphorylation status of SRC in various cell types and tissues, providing valuable insights into its function in normal and disease states. By targeting phospho-SRC (Y416), researchers can uncover novel therapeutic targets for cancer treatment and potentially develop more effective interventions for patients in need.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2534/3055/3932/4067/6714/7525
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3-Src cells, using Phospho-SRC Family-Y416 Rabbit pAb (CABP0480).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
