The Phospho-STAT3-S727 Monoclonal Antibody (CABP0715) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit monoclonal technology, specifically targets the phosphorylated form of Stat3 at the S727 residue, making it an essential tool for studies focusing on the activation and regulation of Stat3 signaling pathways.The antibody is highly specific to human samples and has been validated for use in Western blot applications, offering researchers a reliable method for detecting and analyzing phosphorylated Stat3 levels in different cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STAT3-S727 Monoclonal Antibody
SKU:
CABP0715
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0150
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PMSP R
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:2000 - 1:10000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APRF, Phospho-STAT3-S727
Positive Sample:
HeLa, NIH/3T3, HeLa treated with λ-PP, NIH/3T3 treated with λ-PP
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
88kDa
Observed MW:
100kDa
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome.
Purification Method
Affinity purification
Gene ID
6774
RRID
AB_2863812
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-STAT3-S727 Rabbit mAb (CABP0715) at 1:10000 dilution incubated overnight at 4℃. HeLa cells and NIH/3T3 cells were treated with λPP (2 U/μL) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-S727 Rabbit mAb (CABP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon using Phospho-STAT3-S727 Rabbit mAb (CABP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-STAT3-S727 Rabbit mAb (CABP0715) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
