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Phospho-Tau (T181) Quantitative ELISA

SKU:
SBRS2027
Product Type:
ELISA
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
ELISA Type:
Competitive ELISA
€699
Frequently bought together:

Description

system_update_altDatasheet

Phospho-Tau (T181) Quantitative ELISA - Kit Data

Product SKU: SBRS2027
Size: 96T
Application: This ELISA antibody pair detects human, mouse and rat Phospho Tau (T181).
Uniprot: P10636, P10637, P19332
Gene Symbols: MAPT, MAPTL, MTBT1, TAU
Synonyms: Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Target Species: Human, Mouse, Rat
Compatible Sample Types: Cell Lysates, Cerebrospinal Final (CSF), Plasma, Serum, Tissue Lysates
Design Principle: Sandwich-based
Method of Detection: Colorimetric
Quantitative/Semi-Quantitative: Quantitative
Sensitivity: 0.41pM
Storage/Stability: Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at -20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.

Kit Components:

1. Pre-Coated 96-well Strip Microplate
2. Wash Buffer
3. Anti-Phospho Antibody
4. HRP-Conjugated Secondary Antibody
5. Assay Diluent
6. TMB One-Step Substrate
7. Stop Solution
8. Lysis Buffer
9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Phospho-Tau (T181) Quantitative ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 ul of Stop Solution to each well.
11. Read at 450 nm immediately.

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