The Tyrosine Hydroxylase Antibody (CAB0028) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Tyrosine Hydroxylase Antibody
SKU:
CAB0028
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TYH, DYT14, DYT5b, Tyrosine Hydroxylase
Positive Sample:
Mouse brain, Rat brain, Jurkat
Cellular Localization:
Axon, Cytoplasm, Cytoplasmic Side Of Plasma Membrane, Cytoplasmic Vesicle, Cytosol, Dendrite, Mitochondrion, Neuron Projection, Nucleus, Perikaryon, Perinuclear Region Of Cytoplasm, Smooth Endoplasmic Reticulum, Synaptic Vesicle, Terminal Bouton.
Calculated MW:
59kDa
Observed MW:
59kDa/58kDa
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
7054
RRID
AB_2756901
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells using Tyrosine Hydroxylase Rabbit pAb (CAB0028) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of Neuro-2a cells using Tyrosine Hydroxylase Rabbit pAb (CAB0028) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
