The PHPT1 Antibody (CAB13590) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.PHPT1 is a phosphohistidine phosphatase that plays a crucial role in regulating phosphate levels within cells, making it a key player in various cellular processes such as energy metabolism and signal transduction. Dysregulation of PHPT1 has been implicated in diseases such as cancer, osteoporosis, and metabolic disorders, highlighting its importance in human health.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PHPT1 Antibody
SKU:
CAB13590
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PHP, PHP14, CGI-202, HSPC141, HEL-S-132P, PHPT1
Positive Sample:
MCF7, A-549, THP-1
Cellular Localization:
Cytoplasm.
Calculated MW:
14kDa
Observed MW:
13kDa
This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
29085
RRID
AB_2760453
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PHPT1 Rabbit pAb (CAB13590) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
