The PLK1 Monoclonal Antibody (CAB21082) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed through rabbit immunization, offers high specificity and sensitivity for detecting PLK1 in human samples, making it ideal for use in Western blot applications.PLK1, or Polo-like kinase 1, plays a crucial role in cell cycle progression and is often overexpressed in cancer cells, promoting uncontrolled cell growth. By targeting PLK1, researchers hope to develop more effective cancer treatments that inhibit tumor cell proliferation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Monkey samples.
Product Name:
PLK1 Monoclonal Antibody
SKU:
CAB21082
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Monkey
Clone Number:
ARC52311
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.
Purification Method
Affinity purification
Gene ID
5347
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of various cell lines, using PLK1 Rabbit mAb (CAB21082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using PLK1 Rabbit mAb (CAB21082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
