The PLN Antibody (CAB17964) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it an ideal choice for Western blot applications.PLN, also known as phospholamban, is a critical regulator of the sarcoplasmic reticulum calcium ATPase (SERCA) pump in cardiac muscle cells. Dysregulation of PLN has been linked to heart failure and other cardiac diseases, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PLN Antibody
SKU:
CAB17964
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PLB, CMD1P, CMH18, PLN
Positive Sample:
Mouse lung
Cellular Localization:
Endoplasmic Reticulum, Mitochondrial Membrane, Mitochondrion, Perinuclear Region Of Cytoplasm.
Calculated MW:
6kDa
Observed MW:
12kDa
The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.
Purification Method
Affinity purification
Gene ID
5350
RRID
AB_2861766
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PLN Rabbit pAb (CAB17964) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
