The POC1A Antibody (CAB7465) is a high-quality antibody developed for reliable detection and analysis of target proteins. POC1A is a protein involved in centriole biogenesis and cell cycle regulation, making it a key target for studies in cell biology and cancer research.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the POC1A protein, researchers can accurately and efficiently study its function in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
POC1A Antibody
SKU:
CAB7465
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PIX2, SOFT, WDR51A, POC1A
Positive Sample:
HL-60, NCI-H460, SKOV3, Raji
Cellular Localization:
Cytoplasm, Centriole, Centrosome, Cilium Basal Body, Cytoskeleton, Microtubule Organizing Center, Spindle Pole.
Calculated MW:
45kDa
Observed MW:
45kDa
POC1 proteins contain an N-terminal WD40 domain and a C-terminal coiled coil domain and are part of centrosomes. They play an important role in basal body and cilia formation. This gene encodes one of the two POC1 proteins found in humans. Mutations in this gene result in short stature, onychodysplasia, facial dysmorphism, and hypotrichosis (SOFT) syndrome.
Purification Method
Affinity purification
Gene ID
25886
RRID
AB_2767996
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POC1A Rabbit pAb (CAB7465) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
