The PON1 Polyclonal Antibody (CAB7338) is a research tool designed for studies involving Paraoxonase 1 (PON1), an enzyme known for its antioxidant properties and its role in detoxifying organophosphates. This antibody, raised in rabbits, is highly specific to human PON1 and has been validated for use in Western blot applications. By binding to the PON1 protein, researchers can easily detect and analyze its expression in a variety of cell types, making it a valuable tool for investigations in toxicology, cardiovascular disease, and oxidative stress research.
PON1 is a key player in protecting against oxidative stress and lipid peroxidation, making it a crucial enzyme in maintaining proper vascular health and reducing the risk of cardiovascular diseases. Its ability to detoxify harmful compounds also makes it a potential target for therapeutic interventions in conditions involving pesticide exposure or inflammation-related diseases. Understanding the function and regulation of PON1 can provide valuable insights into developing strategies for preventing and treating these health issues.
Product Name:
PON1 Rabbit Polyclonal Antibody
SKU:
CAB7338
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human PON1 (NP_000437.3).
This gene encodes a member of the paraoxonase family of enzymes and exhibits lactonase and ester hydrolase activity. Following synthesis in the kidney and liver, the enzyme is secreted into the circulation, where it binds to high density lipoprotein (HDL) particles and hydrolyzes thiolactones and xenobiotics, including paraoxon, a metabolite of the insecticide parathion. Polymorphisms in this gene may be associated with coronary artery disease and diabetic retinopathy. The gene is found in a cluster of three related paraoxonase genes on chromosome 7.
Purification Method:
Affinity purification
Gene ID:
5444
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using PON1 Rabbit pAb (CAB7338) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
