Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit
The Porcine Bone Morphogenetic Protein-4 (BMP4) ELISA Kit is specifically designed for the accurate detection of BMP4 levels in porcine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an essential tool for researchers in various fields.BMP4 is a key player in bone formation and development, regulating the differentiation of various cell types and influencing tissue regeneration.
Its role in embryonic development and adult tissue homeostasis makes it a valuable biomarker for studying skeletal disorders, developmental biology, and regenerative medicine.Whether investigating bone morphogenesis, tissue repair, or stem cell differentiation, the Porcine BMP4 ELISA Kit offers a precise and efficient solution for researchers looking to explore the functions and therapeutic potential of BMP4 in porcine model systems.
Product Name:
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit
Product Code:
PRDL00020
Size:
96 Assays
Target:
BMP4
Synonyms:
BMP2B, BMP2-B, BMP2B1, ZYME, DVR4, Bone morphogenetic protein 2B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
