The Porcine CRP (C-Reactive Protein) ELISA Kit serves as a specialized assay designed for the precise and quantitative measurement of C-Reactive Protein (CRP) levels in various biological samples sourced from porcine models.
CRP is a key biomarker of inflammation, extensively involved in numerous cellular functions such as immune response modulation, cell differentiation, and associated cascades of biological pathways. Its role in acute-phase responses and inflammatory conditions underscores its utility in understanding disease progression, immune processes, and responses to infections in porcine systems. With a commitment to delivering accurate results, the Porcine CRP ELISA Kit provided by Assay Genie offers unparalleled sensitivity and specificity, ensuring the reproducibility and dependability of experimental outcomes. Manufactured under strict quality control measures, this ELISA kit is engineered to deliver consistent and robust performance, making it an ideal choice for researchers conducting studies related to porcine inflammatory responses, immune system dynamics, and disease pathology. Its user-friendly design further enhances its suitability for a wide range of research applications, empowering scientists with a valuable tool to explore the role of CRP in porcine health and disease states.
Product Name:
Porcine CRP (C-Reactive Protein) ELISA Kit
SKU:
AEES00274
Target:
Porcine CRP (C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine CRP. You can calculate the concentration of Porcine CRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
96-107
93-106
Average (%)
93
102
99
1:4
Range (%)
90-104
83-93
84-97
Average (%)
95
88
89
1:8
Range (%)
91-103
84-98
85-96
Average (%)
98
89
90
1:16
Range (%)
88-103
83-95
85-96
Average (%)
95
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
98
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
250.06
614.1
1916.22
226.14
552.85
1907.04
Standard deviation
14.15
30.4
104.82
11.44
30.19
61.22
C V (%)
5.66
4.95
5.47
5.06
5.46
3.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine CRP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine CRP in samples. No significant cross-reactivity or interference between Porcine CRP and analogues was observed.
