This kit is comprised by HRP conjugate, other reagents and ELISA Microtiter plate pre-coated with recombinant Porcine Parvovirus (PPV) antigen. Apply the principle of enzyme-linked immunoassay(ELISA) to detect PPV-Ab in serum of porcine. During the experiment, add control and samples into the ELISA Microtiter plate, PPV-Ab will be bound with the antigen on the ELISA Microtiter plate. Then horseradish peroxidase (HRP) conjugate is added to each ELISA microtiter plate wells, and substrate reagent is added for color development. There is a positive correlation between the OD value of samples and the concentration of PPV-Ab. Measure the absorbance value of each well by using a microplate reader with 450 nm (630 nm) wavelength, then we can judge whether PPV antibody exist in the sample.
