The Porcine VWF (Von Willebrand Factor) ELISA Kit is a specialized assay meticulously designed for the precise and quantitative determination of Von Willebrand Factor (VWF) levels in porcine biological samples. VWF plays a critical role in the process of blood coagulation and platelet adhesion, making it essential for hemostasis and vascular health in pigs. This ELISA kit enables researchers to accurately measure VWF concentrations in porcine samples, providing valuable insights into the coagulation cascade, blood clotting mechanisms, and vascular conditions in pigs. By evaluating VWF levels, researchers can deepen their understanding of hemostatic processes and vascular disorders in porcine models, aiding in the development of targeted interventions for blood-related issues.
With exceptional sensitivity and specificity, the Porcine VWF ELISA Kit ensures precise and reproducible results, empowering researchers to explore the role of VWF in pig health and disease. Manufactured under stringent quality standards, this kit offers robust performance and user-friendly protocols, making it an ideal choice for impactful research in porcine hemostasis and vascular biology studies.
Product Name:
Porcine VWF (Von Willebrand Factor) ELISA Kit
SKU:
AEES00717
Target:
Porcine VWF (Von Willebrand Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine VWF. You can calculate the concentration of Porcine VWF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
88-104
86-102
Average (%)
105
95
93
1:4
Range (%)
101-113
91-103
83-95
Average (%)
107
96
90
1:8
Range (%)
92-107
87-97
89-100
Average (%)
100
92
94
1:16
Range (%)
95-113
86-97
87-101
Average (%)
103
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
102
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.35
8.95
46.74
5.47
9.17
49.76
Standard deviation
0.3
0.49
1.81
0.34
0.5
2.7
C V (%)
5.61
5.47
3.87
6.22
5.45
5.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine VWF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine VWF in samples. No significant cross-reactivity or interference between Porcine VWF and analogues was observed.
