The POU6F2 Polyclonal Antibody (PAC020265) is a valuable tool for researchers studying the POU6F2 transcription factor, which plays a key role in gene regulation and cell differentiation. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for a range of applications including Western blotting and immunohistochemistry.POU6F2 is known to be involved in various biological processes, including embryonic development, organogenesis, and neuronal differentiation. Dysregulation of POU6F2 has been linked to diseases such as cancer, developmental disorders, and neurological conditions.
By targeting POU6F2 with this antibody, researchers can gain valuable insights into the molecular mechanisms underlying these diseases.With its ability to specifically recognize and bind to POU6F2, this antibody is an essential tool for investigators looking to unravel the role of this transcription factor in health and disease. Its reliable performance and versatility make it a valuable asset in the fields of molecular biology, developmental biology, and medical research.
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO20265(POU6F2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane: Mouse heart tissue, Primary antibody: PACO20265(POU6F2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO20265(POU6F2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition.
Synonyms:
POU class 6 homeobox 2
UniProt Protein Function:
POU6F2: Probable transcription factor likely to be involved in early steps in the differentiation of amacrine and ganglion cells. Recognizes and binds to the DNA sequence 5'-ATGCAAAT-3'. Isoform 1 does not bind DNA. Defects in POU6F2 are a cause of hereditary susceptibility to Wilms tumor 5 (WT5). WT5 is a pediatric malignancy of kidney and one of the most common solid cancers in childhood. Belongs to the POU transcription factor family. Class- 6 subfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-binding; Cell development/differentiation; Transcription factorChromosomal Location of Human Ortholog: 7p14.1Cellular Component: nucleusMolecular Function: transcription factor activityBiological Process: central nervous system development; ganglion mother cell fate determination; regulation of transcription, DNA-dependent; visual perceptionDisease: Wilms Tumor 5
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
