The PP2A-B55alpha/PR55alpha/PPP2R2A Monoclonal Antibody (CAB24261) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in mice, is highly specific and reacts with human samples, making it ideal for use in Western blot applications. It targets the PP2A B55 subunit, enabling precise detection and analysis in a variety of cell types.PP2A is a major serine/threonine phosphatase that regulates various cellular processes, including cell cycle progression, apoptosis, and signal transduction. The B55 regulatory subunit is involved in controlling PP2A activity and substrate specificity, making it a key player in cellular signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
5520
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using PP2A-B55α/PR55α/PPP2R2A Rabbit mAb (CAB24261) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse brain using PP2A-B55α/PR55α/PPP2R2A Rabbit mAb (CAB24261) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
