The [KO Validated] PKA RIIalpha (PRKAR2A) Antibody (CAB1531) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It specifically binds to the PRKAR2A protein, enabling accurate detection and analysis in various cell types, making it ideal for studies in molecular biology and signal transduction research.PRKAR2A, also known as cAMP-dependent protein kinase regulatory subunit, is a key component of the protein kinase A (PKA) signaling pathway, which regulates a wide range of cellular processes including metabolism, gene expression, and cell growth.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] PKA RIIalpha (PRKAR2A) Antibody
SKU:
CAB1531
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PKR2, PRKAR2, A)
Positive Sample:
HeLa, Jurkat, SW480, LO2, Mouse lung, Rat skeletal muscle
Cellular Localization:
Cell Membrane, Cytoplasm.
Calculated MW:
46kDa
Observed MW:
46kDa
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Purification Method
Affinity purification
Gene ID
5576
RRID
AB_2861713
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (CAB1531) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Western blot analysis of various lysates using [KO Validated] PKA RIIα (PRKAR2A) Rabbit pAb (CAB1531) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
