The PRMT1 Monoclonal Antibody (CAB4502) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting PRMT1 in human samples, making it ideal for use in Western blot and immunohistochemistry applications.PRMT1 plays a crucial role in various cellular processes, including chromatin remodeling, RNA processing, and signal transduction, making it a target of interest in cancer research and other fields. The PRMT1 Rabbit Monoclonal Antibody enables accurate detection and analysis of PRMT1 expression levels in different cell types, providing valuable insights into its function and potential implications in disease.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PRMT1 Monoclonal Antibody
SKU:
CAB4502
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1066
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ANM1, HCP1, IR1B4, HRMT1L2, PRMT1
Positive Sample:
SW620, A549
Cellular Localization:
Cytoplasm, Nucleus, Cytosol, Nucleoplasm.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Purification Method
Affinity purification
Gene ID
3276
RRID
AB_2863283
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PRMT1 Rabbit mAb (CAB4502) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using PRMT1 Rabbit mAb (CAB4502) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using PRMT1 Rabbit mAb (CAB4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using PRMT1 Rabbit mAb (CAB4502) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
