The PSMB10 Monoclonal Antibody (CAB21123) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using hybridoma technology, offers high specificity and sensitivity in detecting PSMB10 in various experimental settings, including Western blot and immunohistochemistry.PSMB10 is a key component of the immunoproteasome, an alternative form of the proteasome involved in generating peptides for major histocompatibility complex (MHC) class I antigen presentation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PSMB10 Monoclonal Antibody
SKU:
CAB21123
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2999
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LMP10, MECL1, PRAAS5, beta2i, PSMB10
Positive Sample:
Raji, Mouse liver, Rat lung, Rat spleen, Rat thymus
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
29kDa
Observed MW:
29kDa
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
Purification Method
Affinity purification
Gene ID
5699
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PSMB10 Rabbit mAb (CAB21123) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): 293T. Exposure time: 30s.
Western blot analysis of lysates from Mouse liver, using PSMB10 Rabbit mAb (CAB21123) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
