The QuickStep Mouse PG Progesterone ELISA Kit is a cutting-edge assay designed for the quantitative measurement of progesterone levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for a variety of research applications.Progesterone is a key hormone involved in the regulation of the female reproductive system, including menstrual cycle regulation, pregnancy support, and embryo development. Dysregulation of progesterone levels can lead to fertility issues, pregnancy complications, and other reproductive disorders.
This ELISA kit enables researchers to study the role of progesterone in these processes and develop potential therapeutic interventions.Whether investigating reproductive health, hormone-related disorders, or drug development, the QuickStep Mouse PG Progesterone ELISA Kit is an essential tool for researchers looking to advance their understanding of progesterone biology and its implications for human health.
Product Name:
QuickStep Mouse Progesterone ELISA Kit
Product Code:
QSES037
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Mouse
Sensitivity:
24.97 pg/mL
Range:
78.13-5000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Mouse serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Pg are added to the micro ELISA plate wells. Mouse Pg in samples (or standards) competes with a fixed amount of Mouse Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Mouse Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Mouse Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
272.69
578.76
1903.79
255.63
574.20
1978.04
Standard deviation
17.59
27.09
59.97
17.74
30.83
98.51
CV (%)
6.45
4.68
3.15
6.94
5.37
4.98
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
91-104
97
EDTA plasma(n=8)
91-101
96
Samples were spiked with high concentrations of Mouse Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
