The QuickStep Rat IgG (Immunoglobulin G) ELISA Kit is a highly reliable and accurate tool for detecting levels of IgG in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it perfect for a wide range of research purposes.IgG is a critical component of the immune system, playing a vital role in fighting off infections and providing immunity.
Monitoring IgG levels can help researchers understand immune responses, autoimmune diseases, and allergic reactions in rats, making this kit an essential tool for studying these conditions and developing potential treatments.Overall, the QuickStep Rat IgG ELISA Kit is a valuable resource for researchers looking to analyze and quantify IgG levels in rat samples with precision and accuracy.
Product Name:
QuickStep Rat IgG ELISA Kit
Product Code:
QSES052
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
2.98 ng/mL
Range:
7.81-500 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
IgG
Application:
This ELISA kit applies to the in vitro quantitative determination of IgG concentrations in serum and plasma.
Specificity:
This kit recognizes IgG in samples. No significant cross-reactivity or interference between IgG and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 20000-80000 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IgG are added to the micro ELISA plate wells. Rat IgG in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IgG. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat IgG. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
23.38
68.80
230.89
21.47
74.91
214.43
Standard deviation
1.42
3.51
7.37
1.34
3.26
6.65
CV (%)
6.08
5.10
3.19
6.26
4.35
3.10
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-102
94
EDTA plasma(n=8)
85-97
90
Samples were spiked with high concentrations of Rat IgG and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
