The QuickStep Sheep PG (Progesterone) Elisa Kit is specifically designed for the precise measurement of progesterone levels in sheep serum, plasma, and other biological samples. This kit provides exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results for various research purposes.Progesterone is a vital hormone in sheep reproduction, playing a key role in regulating the estrous cycle and supporting pregnancy.
Monitoring progesterone levels is essential for managing breeding programs, detecting pregnancy, and optimizing fertility in sheep. The QuickStep Sheep PG Elisa Kit offers researchers a valuable tool for studying reproductive health in sheep and developing effective management strategies for livestock breeding.
Product Name:
QuickStep Sheep Progesterone ELISA Kit
Product Code:
QSES062
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Sheep
Sensitivity:
0.06 ng/mL
Range:
0.16-10 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Sheep serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep Pg are added to the micro ELISA plate wells. Sheep Pg in samples (or standards) competes with a fixed amount of Sheep Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Sheep Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Sheep Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
0.54
1.24
4.88
0.53
1.17
4.87
Standard deviation
0.03
0.07
0.16
0.03
0.06
0.15
CV (%)
5.04
5.36
3.24
5.59
4.96
3.12
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-101
94
EDTA plasma(n=8)
88-100
93
Samples were spiked with high concentrations of Sheep Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
