The RAB5C Polyclonal Antibody (CAB21583) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high specificity and sensitivity when detecting Rab5c protein in human samples, making it ideal for use in Western blot applications.Rab5c is known to play a crucial role in the endocytic pathway, specifically in the early stages of endosome maturation. Dysregulation of Rab5c has been implicated in various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RAB5C Polyclonal Antibody
SKU:
CAB21583
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RABL, L1880, RAB5L, RAB5CL, RAB5C
Positive Sample:
A549
Cellular Localization:
Cell Membrane, Cytoplasmic Side, Early Endosome Membrane, Lipid-Anchor, Melanosome.
Calculated MW:
23kDa
Observed MW:
23kDa
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).
Purification Method
Affinity purification
Gene ID
5878
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A549 cells using RAB5C Rabbit pAb (CAB21583) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
