The Rabbit IgA (Immunoglobulin A) ELISA Kit is specifically developed for the sensitive and quantitative measurement of Immunoglobulin A (IgA) levels in various biological samples from rabbits. IgA plays a crucial role in mucosal immunity, defending against infections and maintaining immune homeostasis in the body.
Accurate measurement of IgA is essential for understanding the immune response and investigating immune-related disorders in rabbits. This ELISA kit offers outstanding sensitivity and specificity, ensuring precise and reproducible results in the analysis of rabbit IgA levels. Manufactured under stringent quality control standards, the kit ensures robust performance and user-friendly protocols, making it an ideal choice for researchers studying rabbit immunology and infectious diseases.
Product Name:
Rabbit IgA (Immunoglobulin A) ELISA Kit
SKU:
AEES00579
Target:
Rabbit IgA (Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IgA. You can calculate the concentration of Rabbit IgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
91-104
98-113
Average (%)
96
98
104
1:4
Range (%)
92-109
87-99
88-102
Average (%)
99
92
94
1:8
Range (%)
88-100
86-98
84-98
Average (%)
95
92
91
1:16
Range (%)
91-102
81-96
82-96
Average (%)
96
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.51
63.27
190.99
19.23
60.69
177.24
Standard deviation
1.14
2.83
6.05
1.11
3.57
6.22
C V (%)
6.16
4.47
3.17
5.77
5.88
3.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IgA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IgA in samples. No significant cross-reactivity or interference between Rabbit IgA and analogues was observed.
