**** The Rabbit MMP-9 (Matrix Metalloproteinase 9) ELISA Kit is a specialized assay designed for the quantitative detection of Matrix Metalloproteinase 9 (MMP-9) levels in various biological samples from rabbits.
MMP-9 is a crucial enzyme involved in extracellular matrix degradation and remodeling processes, making it essential for tissue repair, angiogenesis, and immune response modulation in rabbit models. Accurate measurement of MMP-9 levels is paramount for understanding its role in various physiological and pathological conditions, particularly in tissue remodeling, wound healing, and inflammatory responses in rabbits. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for assessing MMP-9 concentrations. Manufactured under stringent quality control standards, this kit delivers robust performance and straightforward protocols, making it an ideal choice for research studies focusing on matrix metalloproteinases and tissue dynamics in rabbits.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit MMP-9. You can calculate the concentration of Rabbit MMP-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
88-98
93-105
Average (%)
90
93
100
1:4
Range (%)
85-98
85-95
89-103
Average (%)
92
90
96
1:8
Range (%)
81-94
84-97
92-107
Average (%)
87
91
100
1:16
Range (%)
80-92
87-101
93-106
Average (%)
86
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.79
29.06
70.94
10.59
31.73
74.19
Standard deviation
0.75
1.4
3.76
0.68
1.72
2.88
C V (%)
6.95
4.82
5.3
6.42
5.42
3.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit MMP-9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit MMP-9 in samples. No significant cross-reactivity or interference between Rabbit MMP-9 and analogues was observed.
