Rabbit S100 Calcium Binding Protein B (S100B) ELISA Kit
The Rabbit S100 Calcium Binding Protein B (S100B) ELISA Kit is specifically designed for the precise and accurate measurement of S100B levels in rabbit serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and selectivity, ensuring dependable and consistent results for a variety of research purposes. S100B is a key calcium-binding protein that plays a critical role in various cellular processes, including cell growth, differentiation, and inflammation.
It is particularly important in neurodegenerative diseases, traumatic brain injury, and psychiatric disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With the Rabbit S100 Calcium Binding Protein B (S100B) ELISA Kit, researchers can confidently measure S100B levels with precision and accuracy, leading to a deeper understanding of the role this protein plays in various diseases and the development of targeted therapies.
Product Name:
Rabbit S100 Calcium Binding Protein B (S100B) ELISA Kit
Product Code:
RBDL00066
Size:
96 Assays
Target:
S100B
Synonyms:
S100-B, NEF, S100Beta, S-100 protein subunit beta
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
