The Raptor Monoclonal Antibody (CAB8992) is a high-quality antibody developed for reliable detection and analysis of target proteins. This high-quality antibody, produced in rabbits, exhibits exceptional reactivity with human samples and has been rigorously validated for use in Western blot analyses. By specifically binding to the CD300A protein, this antibody enables precise detection and analysis across a variety of cell types, making it an indispensable resource for investigations in immunology and cancer research.CD300A, also recognized as an immune inhibitory receptor, serves as a pivotal player in maintaining immune balance by modulating inflammation and suppressing allergic responses.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Raptor Monoclonal Antibody
SKU:
CAB8992
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1375
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KOG1, Mip1, Raptor
Positive Sample:
293T, RD, Mouse brain
Cellular Localization:
Cytoplasm, Cytoplasmic Granule, Lysosome.
Calculated MW:
149kDa
Observed MW:
149kDa
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
57521
RRID
AB_2863638
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Raptor Rabbit mAb (CAB8992) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Raptor Rabbit mAb (CAB8992) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
