The Rat Adrenocorticotropic Hormone (ACTH) ELISA Kit is meticulously crafted for the precise and quantitative measurement of ACTH levels in various rat biological samples. ACTH, a pivotal hormone produced by the pituitary gland, plays a critical role in regulating cortisol secretion from the adrenal glands. This hormone is essential for the body's stress response, metabolism, and overall homeostasis. This state-of-the-art ELISA kit empowers researchers to explore the dynamics of ACTH levels, providing valuable insights into its role in stress responses, adrenal function, and potential implications in a range of physiological and pathological conditions.
With exceptional sensitivity and specificity, this assay ensures accurate and reproducible results, offering a reliable platform for investigating ACTH biology. Manufactured under stringent quality control standards, this kit delivers robust performance, making it a reliable and user-friendly tool for scientific research endeavors.
Product Name:
Rat ACTH (Adrenocorticotropic Hormone) ELISA Kit
SKU:
AEES00308
Target:
Rat ACTH (Adrenocorticotropic Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACTH. During the reaction, ACTH in the sample or standard competes with a fixed amount of ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
95-109
85-95
Average (%)
103
101
90
1:4
Range (%)
86-99
90-104
101-114
Average (%)
91
97
107
1:8
Range (%)
83-96
90-103
93-108
Average (%)
90
95
101
1:16
Range (%)
92-104
88-100
97-114
Average (%)
97
94
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
86-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.7
99.8
387.8
48.2
101.9
401.4
Standard deviation
3.1
5.3
15.9
3.1
5.2
14.5
C V (%)
6.11
5.31
4.1
6.43
5.1
3.61
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ACTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed.
